Col1a1^{tm2(CAG-EGFR*)Char}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm2(CAG-EGFR*)Char}
• Name: collagen, type I, alpha 1; targeted mutation 2, Al Charest
• MGI ID: MGI:3837826
• Synonyms: Col1alpha1-EGFR^vIII
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm2(CAG-EGFR*)Char} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:146494
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, No functional change)
• Mutation: Insertion
• Col1a1^{tm2(CAG-EGFR*)Char} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  EGFR (1956)

  Egfr	(MGI:95294)

• Mutation details: A CAG promoter was used to drive expression the oncogenic variant vIII of human EGFR with an intervening floxed STOP cassette inserted into the 3' untranslated region. The mutant form contains an in-frame deletion of exons 2 through 7 and codes for a ligand independent receptor. (J:146494)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

References

• Original: J:146494 Zhu H, et al., Oncogenic EGFR signaling cooperates with loss of tumor suppressor gene functions in gliomagenesis. Proc Natl Acad Sci U S A. 2009 Feb 24;106(8):2712-6
• All: 5 reference(s)