Hsp90b1^tm1Yarg
Targeted Allele Detail

Summary

• Symbol: Hsp90b1^tm1Yarg
• Name: heat shock protein 90, beta (Grp94), member 1; targeted mutation 1, Yair Argon
• MGI ID: MGI:3837939
• Gene: Hsp90b1 Location: Chr10:86526705-86541308 bp, - strand Genetic Position: Chr10, 43.05 cM
• Alliance: Hsp90b1^tm1Yarg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:146397
• Parent Cell Line: C1 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neo cassette in inverse orientation was inserted in exon 3 via homologous recombination. This insertion created a stop at codon 62. RT-PCR and immunoblot analysis confirmed the absence of transcript and protein expression in homozygous embryos. (J:146397)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hsp90b1 Mutation: 35 strains or lines available

References

• Original: J:146397 Wanderling S, et al., GRP94 is essential for mesoderm induction and muscle development because it regulates insulin-like growth factor secretion. Mol Biol Cell. 2007 Oct;18(10):3764-75
• All: 1 reference(s)