Numa1^tm1Dwc
Targeted Allele Detail

Summary

• Symbol: Numa1^tm1Dwc
• Name: nuclear mitotic apparatus protein 1; targeted mutation 1, Don W Cleveland
• MGI ID: MGI:3838104
• Synonyms: NuMA^Neo
• Gene: Numa1 Location: Chr7:101583318-101664171 bp, + strand Genetic Position: Chr7, 54.7 cM
• Alliance: Numa1^tm1Dwc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:146214
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted (Conditional ready, Hypomorph) Mutation: Insertion Mutation details: A loxP site was inserted upstream of exon 22, and an frt flanked neo cassette with a 5' loxP site was inserted downstream of exon 22. Reduced protein expression was confirmed by western blot analysis. This allele is hypomorphic. (J:146214)

Generation of the Numa1^tm1Dwc, Numa1^tm1.1Dwc, and Numa1^tm1.2Dwc alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Numa1 Mutation: 78 strains or lines available

References

• Original: J:146214 Silk AD, et al., Requirements for NuMA in maintenance and establishment of mammalian spindle poles. J Cell Biol. 2009 Mar 9;184(5):677-90
• All: 1 reference(s)