Azin1^{Gt(Ayu17-689)Imeg}
Gene trapped Allele Detail

Summary

• Symbol: Azin1^{Gt(Ayu17-689)Imeg}
• Name: antizyme inhibitor 1; gene trap Ayu17-689, Institute of Molecular Embryology and Genetics
• MGI ID: MGI:3838345
• Gene: Azin1 Location: Chr15:38487671-38519510 bp, - strand Genetic Position: Chr15, 15.17 cM, cytoband C
• Alliance: Azin1^{Gt(Ayu17-689)Imeg} page

Mutation origin

• Mutant Cell Line: Ayu17-689
• Germline Transmission: Earliest citation of germline transmission: J:146570
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutations: Insertion of gene trap vector, Intragenic deletion
• Mutation details: The gene trap vector, pU-17, integrated at a single site in the mutant cell line. This vector is composed of a mouse En2 splicing acceptor, a lox71 site, beta galactosidase, neomycin, loxP, a poly A site, and lox2272. The integrated trapping vector was found to have a 522 bp deletion at its 5' terminus. Vector insertion was in intron 2 about 9.295 kb upstream of exon3 in which the translational start codon is located. Northern blot analysis demonstrated that gene expression was inhibited in heterozygotes and strongly suppressed in homozygotes. (J:146570)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Azin1 Mutation: 27 strains or lines available

References

• Original: J:146570 Tang H, et al., Role of ornithine decarboxylase antizyme inhibitor in vivo. Genes Cells. 2009 Jan;14(1):79-87
• All: 1 reference(s)