Tg(Wfs1-cre/ERT2)3Aibs
Transgene Detail

Summary

• Symbol: Tg(Wfs1-cre/ERT2)3Aibs
• Name: transgene insertion 3, Ed Lein
• MGI ID: MGI:3838496
• Synonyms: Wfs1-Tg3-CreERT2
• Transgene: Tg(Wfs1-cre/ERT2)3Aibs Location: unknown
• Alliance: Tg(Wfs1-cre/ERT2)3Aibs page

Transgene origin

• Strain of Origin: (C57BL/6 x C3H)F1 x C57BL/6

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Wfs1-cre/ERT2)3Aibs expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Wfs1 (MGI:1328355)

• Mutation details: The 196 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-405O19, containing the entire Wsf1 locus (and other genes), was modified by targeting a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand-binding domain), SV40 polyA signal, and frt-flanked neomycin cassette to the ATG start site of the Wsf1 locus. The targeted BAC sequences were further modified using FLP recombination to remove the selection cassette and linearized to remove its original vector backbone. Cre activity is directed to the cortex, hippocampus, striatum, thalamus, and cerebellum only following tamoxifen administration. (J:146821, J:155793)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Wfs1 (mouse) Summary of all recombinase alleles driven by Wfs1. Comparative matrix view of recombinase activities Alliance mouse Wfs1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:155793 Madisen L, et al., A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci. 2010 Jan;13(1):133-40
• All: 17 reference(s)