Drinkcacl22^PWK/PhJ
QTL Variant Detail

Summary

• QTL variant: Drinkcacl22^PWK/PhJ
• Name: drink calcium chloride 2 2; PWK/PhJ
• MGI ID: MGI:3838583
• QTL: Drinkcacl22 Location: unknown Genetic Position: Chr16, Syntenic

Variant origin

• Strain of Specimen: PWK/PhJ

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased calcium chloride preference and intake compared to C57BL/6J. (J:144262)
• Inheritance: Other (see notes)

Notes

Drinkcacl22 exhibits additive inheritance.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:144262

Linkage analysis was performed on (C57BL/6J x PWK/PhJ)F2 animals to identify genetic loci associated with preference and intake of calcium and other salt compounds. Animals were subjected to a two-bottle choice test between water and one other solution (CaCl2, CaLa, MgCl2, KCl, NH4Cl, NaCl, citric acid, QHCl, or saccharin) for a period of 96 hours. PWK/PhJ is a high consumer of calcium chloride (CaCl2) and calcium lactate (CaLa) whereas C57BL/6J is a relatively low consumer. PWK/PhJ also consumes more MgCl2 compared to C57BL/6J. However, when consumption of KCl, NaCl and saccharin were analyzed, C57BL/6J animals consumed more of these solutions compared to PWK/PhJ animals. Linkage analysis was performed using 116 polymorphic markers at a resolution of 10cM - 30cM. Thirty QTLs reaching statistical significance were identified. Authors assigned separate QTL nomenclature for each of the salt compound QTL even if they mapped to the same linkage marker.

Significant linkage to intake of calcium chloride (LOD=4.2), calcium lactate (LOD=5.9), and magnesium chloride (LOD=3.3) mapped to 33 Mb on mouse Chromosome 16 near D16Mit60. This locus was assigned three QTL symbols: Drinkcacl22 (drink calcium chloride 2 2), Drinkcala2 (drink calcium lactate 2) and Drinkmgcl22 (drink magnesium chloride 2 2). Casr (26.3 cM; 36.49 Mb) is considered a possible candidate gene for these QTL. PWK/PhJ-derived alleles at this locus confers increased intake of calcium chloride, calcium lactate and magnesium chloride with an additivemode of inheritance. Drinkcacl22 and Drinkcala2 also display significant linkage to calcium chloride and calcium lactate preference with LOD=3.5 and LOD=3.8, respectively. PWK/PhJ-derived alleles at this locus confer increased preference for calcium chloride and calcium lactate with additive inheritance. Drinkcacl22 explains 4.6% of the intake variance and 3.8% of the preference variance for calcium chloride. Drinkcala2 explains 8.1% of the intake variance and 11% of the preference variance for calcium lactate. Drinkmgcl22 explains 3.5% of the intake variance for magnesium chloride.

References

• Original: J:144262 Tordoff MG, et al., Calcium taste preferences: genetic analysis and genome screen of C57BL/6J x PWK/PhJ hybrid mice. Genes Brain Behav. 2008 Aug;7(6):618-28
• All: 1 reference(s)