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Igh-7tm1.1Lam
Targeted Allele Detail
Summary
Symbol: Igh-7tm1.1Lam
Name: immunoglobulin heavy chain 7 (heavy chain of IgE); targeted mutation 1.1, Marinus C Lamers
MGI ID: MGI:3838715
Synonyms: Delta-M1M2
Gene: Igh-7  Location: Chr12:113228142-113241336 bp  Genetic Position: Chr12, Syntenic
Alliance: Igh-7tm1.1Lam page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:146733
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  BALB/c
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion
 
Mutation detailsA point mutation was targeted to the third codon of exon 6 to introduce a stop codon and a frameshift mutation to the portion of the gene that encodes the cytoplasmic tail. LoxP sites were also inserted upstream of exon 5 and downstream of the mutated exon 6. A neomycin/tk selection cassette followed by a third loxP site was inserted further downstream. Transient expression of cre recombinase removed the selection cassette and exons 5 and 6. Correct targeting and recombination were confirmed by Southern blotting. This allele was predicted to encode an IgE heavy chain without transmembrane or cytoplasmic domains. (J:146733)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Igh-7 Mutation:  1 strain or line available
References
Original:  J:146733 Achatz G, et al., Effect of transmembrane and cytoplasmic domains of IgE on the IgE response. Science. 1997 Apr 18;276(5311):409-11
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory