Rxra^tm1.1Pcn
Targeted Allele Detail

Summary

• Symbol: Rxra^tm1.1Pcn
• Name: retinoid X receptor alpha; targeted mutation 1.1, Pierre Chambon
• MGI ID: MGI:3839151
• Synonyms: Rxra^afo
• Gene: Rxra Location: Chr2:27566452-27652969 bp, + strand Genetic Position: Chr2, 19.38 cM
• Alliance: Rxra^tm1.1Pcn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:146761
• Parent Cell Line: H1 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Most of exon 2, all of intron 2 and 102 bp of exon 3, encoding for amino acids 11-132, were deleted in an ES cell line that carried Rxra^tm1Ipc . A floxed hygro cassette (between exons 2 and 4) and a floxed neo cassette (between exons 9 and 10) were removed by Cre mediated recombination. The final mutant allele lacks both transcriptional activating function 1 and 2. Northern and Western blot analysis confirmed the expression of a truncated mRNA and protein in homozygous and heterozygous embryos. Expression levels of both the truncated mRNA and protein are lower than those for the wild-type mRNA and protein. (J:146761)

Expression

In Mice Carrying this Mutation:	6 assay results
In Structures Affected by this Mutation:	11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rxra Mutation: 30 strains or lines available

References

• Original: J:146761 Mascrez B, et al., A transcriptionally silent RXRalpha supports early embryonic morphogenesis and heart development. Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4272-7
• All: 1 reference(s)