Seletm2Hyn
Targeted Allele Detail
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Symbol: |
Seletm2Hyn |
Name: |
selectin, endothelial cell; targeted mutation 2, Richard Hynes |
MGI ID: |
MGI:3839963 |
Synonyms: |
E-selectin- |
Gene: |
Sele Location: Chr1:163875773-163885246 bp, + strand Genetic Position: Chr1, 71.35 cM
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Alliance: |
Seletm2Hyn page
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Germline Transmission: |
Earliest citation of germline transmission:
J:31626
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Parent Cell Line: |
D3 (ES Cell)
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Strain of Origin: |
129S2/SvPas
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Allele Type: |
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Targeted (Null/knockout) |
Mutations: |
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Insertion, Intragenic deletion
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Mutation details: A modified D3 ES cell line heterozygous for the Selptm1Hyn allele was targeted a second time. The exons encoding the signal peptide, lectin domain, and a portion of the epidermal growth factor domain were replaced by the insertion of a PGK-hygro cassette. Both Northern and RT-PCR analysis revealed an absence of normal mRNA for E-selectin but the presence of mRNA for P-selectin in extracts from cardiac and pulmonary tissue of homozygous mutant mice. This targeted mutation occurred in trans with the Selptm1Hyn mutation and were subsequently bred apart.
(J:31626)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:31626 Frenette PS, et al., Susceptibility to infection and altered hematopoiesis in mice deficient in both P- and E-selectins. Cell. 1996 Feb 23;84(4):563-74 |
All: |
15 reference(s) |
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