Selltm2Hyn
Targeted Allele Detail
|
Symbol: |
Selltm2Hyn |
Name: |
selectin, lymphocyte; targeted mutation 2, Richard Hynes |
MGI ID: |
MGI:3839975 |
Synonyms: |
L-selectin- |
Gene: |
Sell Location: Chr1:163889556-163908354 bp, + strand Genetic Position: Chr1, 71.37 cM
|
Alliance: |
Selltm2Hyn page
|
|
Germline Transmission: |
Earliest citation of germline transmission:
J:57973
|
Parent Cell Line: |
D3 (ES Cell)
|
Strain of Origin: |
129S2/SvPas
|
|
Allele Type: |
|
Targeted (Null/knockout) |
Mutations: |
|
Insertion, Intragenic deletion
|
|
|
Mutation details: The three selectin genes, Sele, Sell, and Selp, are found within a 300 kb genomic region. ES cells heterozygote for the Selptm1Hyn and Seletm1Hyn null alleles in cis to each other were targeted a third time. A PGK-puro cassette was used to delete a 2.1 kb region containing the exons encoding the lectin domain and most of the epidermal growth factor domain. Flow cytometric analysis showed an absence of the L-selectin protein but not the two other selectin proteins in leukocytes isolated from homozygotes. Thus, this targeted mutation occurred in trans with the other two null mutations and were subsequently bred apart.
(J:57973)
|
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
|
|
Original: |
J:57973 Robinson SD, et al., Multiple, targeted deficiencies in selectins reveal a predominant role for P-selectin in leukocyte recruitment. Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11452-7 |
All: |
17 reference(s) |
|