Sell^tm4Hyn
Targeted Allele Detail

Summary

• Symbol: Sell^tm4Hyn
• Name: selectin, lymphocyte; targeted mutation 4, Richard Hynes
• MGI ID: MGI:3839979
• Synonyms: L-selectin^-
• Gene: Sell Location: Chr1:163889556-163908354 bp, + strand Genetic Position: Chr1, 71.37 cM
• Alliance: Sell^tm4Hyn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:57973
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The three selectin genes, Sele, Sell, and Selp, are found within a 300 kb genomic region. To generate double knockouts, ES cells heterozygote for the Selp^tm1Hyn and Sele^tm2Hyn null alleles in trans to each other were targeted a third time. A PGK-puro cassette was used to delete a 2.1 kb region containing the exons encoding the lectin domain and most of the epidermal growth factor domain. Flow cytometric analysis of homozygote leukocytes showed an absence of the L-selectin and E-selectin protein while P-selectin express was unaffected. Thus, this targeted mutation occurred in cis with the Sele^tm2Hyn allele and in trans with the Selp^tm1Hyn allele. Due to their genomic proximity, the Sell^tm3Hyn and Sele^tm2Hyn alleles should propagate together through the offspring. (J:57973)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sell Mutation: 42 strains or lines available

References

• Original: J:57973 Robinson SD, et al., Multiple, targeted deficiencies in selectins reveal a predominant role for P-selectin in leukocyte recruitment. Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11452-7
• All: 1 reference(s)