Brd2^{Gt(RRE050)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Brd2^{Gt(RRE050)Byg}
• Name: bromodomain containing 2; gene trap RRE050, BayGenomics
• MGI ID: MGI:3840071
• Synonyms: brd2 lo
• Gene: Brd2 Location: Chr17:34330993-34341581 bp, - strand Genetic Position: Chr17, 17.98 cM, cytoband D
• Alliance: Brd2^{Gt(RRE050)Byg} page

Mutation origin

• Mutant Cell Line: RRE050
• Germline Transmission: Earliest citation of germline transmission: J:147040
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT2Lxf Vector Type: gene trap
• Mutation details: The gene trap vector was inserted into the intron downstream of the first translation initiation site. In situ hybridization confirmed the absence of RNA expression in homozygous embryos. (J:147040, J:159964)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRE050	CC200427 ; (MGI Seq Detail)	5' RACE	Chr17:34337565-34337706 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  15 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	29 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Brd2 Mutation: 45 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:147040 Shang E, et al., Double bromodomain-containing gene Brd2 is essential for embryonic development in mouse. Dev Dyn. 2009 Apr;238(4):908-17
• All: 8 reference(s)