Daxx^tm2Led
Targeted Allele Detail

Summary

• Symbol: Daxx^tm2Led
• Name: Fas death domain-associated protein; targeted mutation 2, Philip Leder
• MGI ID: MGI:3840084
• Synonyms: Dx-cko-Flox
• Gene: Daxx Location: Chr17:34128388-34134564 bp, + strand Genetic Position: Chr17, 17.98 cM
• Alliance: Daxx^tm2Led page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147269
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Intragenic deletion, Single point mutation
• Mutation details: A FRT site flanked targeting vector, with a loxP site at the downstream end and containing a PGK-neo selection cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon I (also called exon 2, when the first non-coding exon is included in the numbering scheme) of the targeted gene, and another loxP site was inserted upstream of exon III (also called exon 4, when the first non-coding exon is included in the numbering scheme). The PGK-neo selection cassette was removed by Flp-recombinase mediated excision. Exon II is flanked by loxP sites. A point mutation within the first loxP site was identified that can interfer with recombination. (J:147269, J:266012)

Tumor Data

List all tumor models in MMHCdb carrying Daxx^tm2Led

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Daxx Mutation: 34 strains or lines available

Notes

Sequence analysis revealed a single base deletion mutation in the first loxP site. When these mutant mice are bred to mice that express Cre recombinase, recombination efficiency is weak and resulting offspring may not have exon 2 deleted in cre-expressing tissue(s). When primary cell cultures derived from this strain (cortical neurons) are infected with lentiviral particles expressing the Cre recombinase, exon II is deleted.

References

• Original: J:147269 McMenamin J, et al., Generation of a Daxx conditional allele. MGI Direct Data Submission. 2009
• All: 7 reference(s)