Pik3cg^tm1Lste
Targeted Allele Detail

Summary

• Symbol: Pik3cg^tm1Lste
• Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma; targeted mutation 1, Len Stephens
• MGI ID: MGI:3840088
• Synonyms: p110gamma^DASAA
• Gene: Pik3cg Location: Chr12:32223472-32258658 bp, - strand Genetic Position: Chr12, 13.74 cM, cytoband B
• Alliance: Pik3cg^tm1Lste page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147259
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Insertion
• Mutation details: Exon 2 was modified to encode 5 amino acid substitutions: T232D, K251A, K254S, K255A, K256A. This also creates an additional NheI restriction site. A floxed neomycin selection cassette was inserted in intron 1. The floxed neo was subsequently excised by crossing to Tg(CMV-cre)1Cgn. Western blot analysis indicates that expression is somewhat reduced and the protein is somewhat insensitive to RAS. (J:147259)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pik3cg Mutation: 59 strains or lines available

References

• Original: J:147259 Suire S, et al., Gbetagammas and the Ras binding domain of p110gamma are both important regulators of PI(3)Kgamma signalling in neutrophils. Nat Cell Biol. 2006 Nov;8(11):1303-9
• All: 4 reference(s)