Pik3r5^tm1Lste
Targeted Allele Detail

Summary

• Symbol: Pik3r5^tm1Lste
• Name: phosphoinositide-3-kinase regulatory subunit 5; targeted mutation 1, Len Stephens
• MGI ID: MGI:3840090
• Synonyms: p101^-
• Gene: Pik3r5 Location: Chr11:68322951-68388675 bp, + strand Genetic Position: Chr11, 41.69 cM
• Alliance: Pik3r5^tm1Lste page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147259
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A BamHI restriction site was engineered at codon 11 in exon 1 and used to insert a beta galactosidase-neomycin selection cassette. This caused the deletion of exons 2 and 3 as well. Neutrophiles have no detectable protein. (J:147259)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pik3r5 Mutation: 49 strains or lines available

References

• Original: J:147259 Suire S, et al., Gbetagammas and the Ras binding domain of p110gamma are both important regulators of PI(3)Kgamma signalling in neutrophils. Nat Cell Biol. 2006 Nov;8(11):1303-9
• All: 3 reference(s)