E2f3^tm2Lees
Targeted Allele Detail

Summary

• Symbol: E2f3^tm2Lees
• Name: E2F transcription factor 3; targeted mutation 2, Jacqueline A Lees
• MGI ID: MGI:3840180
• Synonyms: E2f3a^-
• Gene: E2f3 Location: Chr13:30090558-30170046 bp, - strand Genetic Position: Chr13, 13.09 cM, cytoband A3.3
• Alliance: E2f3^tm2Lees page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147270
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neomycin selection cassette was inserted in exon 1a replacing the two ATG codons and the intervening 34 codons. The neomycin selection cassette was subsequently excised by crossing mice to either B6.FVB-Tg(ACTB-cre)2Mrt or to 129S-Tg(Prm-cre)58Og. Expression of isoform E2f3a is disrupted but isoform E2f3b continues to be expressed. Specific deletion of the "a" isoform was confirmed immunologically. Expression levels of the "b" isoform were not altered. (J:147270)

Tumor Data

List all tumor models in MMHCdb carrying E2f3^tm2Lees

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any E2f3 Mutation: 33 strains or lines available

References

• Original: J:147270 Danielian PS, et al., E2f3a and E2f3b make overlapping but different contributions to total E2f3 activity. Oncogene. 2008 Nov 20;27(51):6561-70
• All: 2 reference(s)