Gt(ROSA)26Sor^{tm1.1(CAG-lacZ,-EGFP)Glh}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(CAG-lacZ,-EGFP)Glh}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, David J Goldhamer
• MGI ID: MGI:3840212
• Synonyms: R26^NZG, R26^ZG
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(CAG-lacZ,-EGFP)Glh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147304
• Parent Cell Line: D1 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6J)F1

Mutation description

Allele Type: Targeted (Reporter) Mutation: Insertion Mutation details: A vector containing a CAG promoter driving expression of a floxed neo/STOP cassette, frt flanked lacZ gene with 3 polyadenylation sequences (secondary STOP cassette) and a nuclear localization signal, and an EGFP gene was inserted into the locus. Germ line, cre mediated removal of the neo/STOP cassette allows for ubiquitous expression of the lacZ gene. The STOP cassette following the lacZ gene remains intact and prevents the expression of EGFP. Expression of EGFP requires flp mediated removal of the secondary STOP cassette. (J:147304)

Generation of Gt(ROSA)26Sor^tm1(CAG-lacZ,-EGFP)Glh, Gt(ROSA)26Sor^tm1.1(CAG-lacZ,-EGFP)Glh, Gt(ROSA)26Sor^tm1.2(CAG-EGFP)Glh, and Gt(ROSA)26Sor^tm1.3(CAG-EGFP)Glh alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 994 strains or lines available

References

• Original: J:147304 Yamamoto M, et al., A multifunctional reporter mouse line for Cre- and FLP-dependent lineage analysis. Genesis. 2009 Feb;47(2):107-14
• All: 9 reference(s)