Gt(ROSA)26Sor^{tm1.2(CAG-EGFP)Glh}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.2(CAG-EGFP)Glh}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.2, David J Goldhamer
• MGI ID: MGI:3840213
• Synonyms: R26^NG
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.2(CAG-EGFP)Glh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147304
• Parent Cell Line: D1 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6J)F1

Mutation description

Allele Type: Targeted (Null/knockout) Mutation: Insertion Mutation details: A vector containing a CAG promoter driving expression of a floxed neo/STOP cassette, frt flanked lacZ gene with 3 polyadenylation sequences (secondary STOP cassette), and an EGFP gene was inserted into the locus. Germ line, flp mediated recombination removes the lacZ/STOP cassette leaving the neo/STOP cassette intact. Cre mediated recombination is necessary to achieve EGFP expression. (J:147304)

Generation of Gt(ROSA)26Sor^tm1(CAG-lacZ,-EGFP)Glh, Gt(ROSA)26Sor^tm1.1(CAG-lacZ,-EGFP)Glh, Gt(ROSA)26Sor^tm1.2(CAG-EGFP)Glh, and Gt(ROSA)26Sor^tm1.3(CAG-EGFP)Glh alleles

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:147304 Yamamoto M, et al., A multifunctional reporter mouse line for Cre- and FLP-dependent lineage analysis. Genesis. 2009 Feb;47(2):107-14
• All: 7 reference(s)