Axin1^tm3Cos
Targeted Allele Detail

Summary

• Symbol: Axin1^tm3Cos
• Name: axin 1; targeted mutation 3, Frank Costantini
• MGI ID: MGI:3840236
• Synonyms: Axin^deltaRGS
• Gene: Axin1 Location: Chr17:26357662-26414785 bp, + strand Genetic Position: Chr17, 13.07 cM
• Alliance: Axin1^tm3Cos page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147293
• Parent Cell Line: CSL3 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: Exon 2 was replaced with a myc-tagged cDNA lacking the RGS domain followed by a floxed neo cassette. Germ line, cre mediated recombination was used to remove the neo cassette. Western blot analysis confirmed 80% expression of the truncated protein. (J:147293)

Expression

In Mice Carrying this Mutation:

3 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Axin1 Mutation: 44 strains or lines available

References

• Original: J:147293 Chia IV, et al., Both the RGS Domain and the Six C-Terminal Amino Acids of Mouse Axin Are Required for Normal Embryogenesis. Genetics. 2009 Apr;181(4):1359-68
• All: 1 reference(s)