Oprm1^tm1Yxp
Targeted Allele Detail

Summary

• Symbol: Oprm1^tm1Yxp
• Name: opioid receptor, mu 1; targeted mutation 1, Ying-Xian Pan
• MGI ID: MGI:3840671
• Gene: Oprm1 Location: Chr10:6708593-6988209 bp, + strand Genetic Position: Chr10, 1.85 cM, cytoband A2
• Alliance: Oprm1^tm1Yxp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147079
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: Exon 11 and part of the adjacent intron, which are located about 30 kb upstream of exon 1, were replaced with an IRES/tau/lacZ-loxP/tk-neo/loxP cassette via homologous recombination. RT-PCR analysis confirmed the absence of transcripts that include exon 11 and indicated about a 25% decrease in overall Oprm1 RNA expression. Receptor binding assays indicated a 21% decrease in binding site density. (J:147079)

Generation of the Oprm1^tm1Yxp allele

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Oprm1 Mutation: 54 strains or lines available

References

• Original: J:147079 Pan YX, et al., Involvement of exon 11-associated variants of the mu opioid receptor MOR-1 in heroin, but not morphine, actions. Proc Natl Acad Sci U S A. 2009 Mar 24;106(12):4917-22
• All: 3 reference(s)