Tcra-Jtm5Krg
Targeted Allele Detail
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| Symbol: |
Tcra-Jtm5Krg |
| Name: |
T cell receptor alpha, joining region; targeted mutation 5, Michael S Krangel |
| MGI ID: |
MGI:3840778 |
| Synonyms: |
56I |
| Gene: |
Tcra-J Location: unknown Genetic Position: Chr14, Syntenic
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| Germline Transmission: |
Earliest citation of germline transmission:
J:147326
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| Parent Cell Line: |
TC1/TC-1 (ES Cell)
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| Strain of Origin: |
129S6/SvEvTac
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| Allele Type: |
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Targeted (Not Applicable) |
| Mutation: |
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Insertion
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Mutation details: A transcriptional terminator element and a frt-flnked neomycin cassette replaced an equivalent amount of genomic DNA downstream of the Tcra-J 56 element. The transcriptional terminator included four polyadenylation sites and 12 lac operators. The operator array was assembled as a tetramer of lac operator trimers with each trimer containing three copies of the sequence TGTGGAATTGTGAGCGCTCACAATTCCACA. Chimeric mice were bred with FLP recombinase mice to remove the neomycin cassette. Germline transcription of the downstream Tcra-J gene segments was reduced to less than 5% of controls in double positive thymocytes isolated from homozygotes on a Rag-null background. This allele differs from the Tcra-Jtm4Krg (56R) allele in that the terminator was inserted with no loss of genomic sequence: in the other (56R), the terminator replaced a genomic DNA segment of equal length.
(J:147326)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Tcra-J Mutation: |
0 strains or lines available
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| Original: |
J:147326 Abarrategui I, et al., Regulation of T cell receptor-alpha gene recombination by transcription. Nat Immunol. 2006 Oct;7(10):1109-15 |
| All: |
1 reference(s) |
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Analysis Tools
