Bptf^tm1.2Cwu
Targeted Allele Detail

Summary

• Symbol: Bptf^tm1.2Cwu
• Name: bromodomain PHD finger transcription factor; targeted mutation 1.2, Carl Wu
• MGI ID: MGI:3841298
• Synonyms: Bptf^deltaExon2
• Gene: Bptf Location: Chr11:106923907-107022953 bp, - strand Genetic Position: Chr11, 70.11 cM
• Alliance: Bptf^tm1.2Cwu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147414
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2, and a frt-flnaked neomycin cassette and a downstream loxP site was inserted downstream of exon 2. Transient cre recombinase expression removed exon 2 and the neomycin cassette. Despite the deletion, mRNA from the locus was still detected in heterozygous tissues including the brain and testis. Sequencing of exons 1-8 revealed the mRNA was out of frame indicating that this is a loss-of-function allele. This was confirmed through allelic complementation assays and by a lack of protein detectable in immunoblot assays. (J:147414)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bptf Mutation: 157 strains or lines available

References

• Original: J:147414 Landry J, et al., Essential role of chromatin remodeling protein Bptf in early mouse embryos and embryonic stem cells. PLoS Genet. 2008 Oct;4(10):e1000241
• All: 1 reference(s)