Bptf^Gt(XG023)Byg
Gene trapped Allele Detail

Summary

• Symbol: Bptf^Gt(XG023)Byg
• Name: bromodomain PHD finger transcription factor; gene trap XG023, BayGenomics
• MGI ID: MGI:3841299
• Synonyms: Bptf^XG023
• Gene: Bptf Location: Chr11:106923907-107022953 bp, - strand Genetic Position: Chr11, 70.11 cM
• Alliance: Bptf^Gt(XG023)Byg page

Mutation origin

• Mutant Cell Line: XG023
• Germline Transmission: Earliest citation of germline transmission: J:147414
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: A beta-geo genetrap vector inserted between exons 15 and 16. This created a truncated Bptf-beta-Geo fusion transcript encoding the N-terminal 1978 residues of the 2903-residue open reading frame, eliminating the conserved glutamine rich region, PHD finger and bromo-domains encoded downstream of exon 15. Fusion transcript was detected by Northern blot in numerous adult heterozygous tissues with highest expression detected in the testis. Allelic complementation assays suggest this is a loss-of-function allele. (J:147414)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XG023	CC200206 ; (MGI Seq Detail)	5' RACE	Chr11:106957954-106963845 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  53 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bptf Mutation: 157 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:147414 Landry J, et al., Essential role of chromatin remodeling protein Bptf in early mouse embryos and embryonic stem cells. PLoS Genet. 2008 Oct;4(10):e1000241
• All: 2 reference(s)