Tg(CAG-ANGPT1*)5Yo
Transgene Detail

Summary

• Symbol: Tg(CAG-ANGPT1*)5Yo
• Name: transgene insertion 5, Yuichi Oike
• MGI ID: MGI:3841702
• Synonyms: CAGp-IND-COMP-Ang1, COMP-Ang1-Tg
• Transgene: Tg(CAG-ANGPT1*)5Yo Location: unknown
• Alliance: Tg(CAG-ANGPT1*)5Yo page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:147388
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(CAG-ANGPT1*)5Yo expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ANGPT1 (284)

  Angpt1

  (MGI:108448)

• Mutation details: A transgene construct was produced in which a modified human Angiopoietin 1 cDNA (COMP-Ang1) was separated from the CAG promoter by a loxP-blasticidineS cDNA-polyA-loxP sequence. The construct was injected into TT2 ES cells. A cre-expressing plasmid was introduced into the transfected ES cells and 2 clones demonstrated high levels of COMP-Ang1 expression. Transgenic mice were generated from clone 5. When crossed to a suitable cre-expressing line, the offspring will express the modified form of human angiopoietin 1. (J:147388)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:147388 Hato T, et al., Angiopoietins contribute to lung development by regulating pulmonary vascular network formation. Biochem Biophys Res Commun. 2009 Apr 3;381(2):218-23
• All: 5 reference(s)