Pou4f1^tm2.1Nat
Targeted Allele Detail

Summary

• Symbol: Pou4f1^tm2.1Nat
• Name: POU domain, class 4, transcription factor 1; targeted mutation 2.1, Jeremy Nathans
• MGI ID: MGI:3842364
• Synonyms: Brn3a^CKOAP, Brn3a^f(AP)
• Gene: Pou4f1 Location: Chr14:104699112-104705435 bp, - strand Genetic Position: Chr14, 54.21 cM, cytoband E1-E3
• Alliance: Pou4f1^tm2.1Nat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147350
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The locus was targeted to have its entire coding region floxed and to express a reporter gene after cre-mediated recombination. A loxP site was inserted in the upstream untranslated region 42 base pairs before the initiator ATG codon. Three repeats of the SV40 early region transcription terminator were added to the untranslated region 48 base pairs downstream of the termination codon, followed by a second loxP site and the coding region of human placental alkaline phosphatase (AP). A frt-flanked neomycin selection cassette followed the AP coding region and was subsequently removed by crossing to mice expressing FLP recombinase. Following cre-mediated recombination, AP activity is detectable in the retina and brain. (J:147350)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pou4f1 Mutation: 13 strains or lines available

References

• Original: J:147350 Badea TC, et al., Distinct roles of transcription factors brn3a and brn3b in controlling the development, morphology, and function of retinal ganglion cells. Neuron. 2009 Mar 26;61(6):852-64
• All: 25 reference(s)