A^tm1Brd
Targeted Allele Detail

Summary

• Symbol: A^tm1Brd
• Name: nonagouti; targeted mutation 1, Allan Bradley
• MGI ID: MGI:3842513
• Gene: a Location: Chr2:154792519-154892932 bp, + strand Genetic Position: Chr2, 76.83 cM
• Alliance: A^tm1Brd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:149352
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (No functional change)
• Mutations: Insertion, Intragenic deletion
• Mutation details: This mutation deletes the 11.8 kb retrotransposon insertion responsible for the nonagouti phenotype of C57BL/6 mice, along with 607 bp of flanking intronic DNA, with retention of a single frt site from an excised frt-flanked selection cassette. It restores expression of the agouti protein in both dorsal and ventral fur. (J:149352)
• Inheritance: Dominant

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1679 strains available Cell Lines: 7671 lines available
• Carrying any a Mutation: 463 strains or lines available

Notes

Although spontaneous revertants of non-agouti retain part of the inserted sequence present in the a allele and are white- or light-bellied agouti (A^w-like), the targeting event that produced A^tm1Brd removed the entire insertion, resulting in a fully agouti coat like that of mice bearing the A allele.

References

• Original: J:149352 Pettitt SJ, et al., Agouti C57BL/6N embryonic stem cells for mouse genetic resources. Nat Methods. 2009 Jun 14;:1-3
• All: 12 reference(s)