Cdkn2a^tm3(cre)Cjs
Targeted Allele Detail

Summary

• Symbol: Cdkn2a^tm3(cre)Cjs
• Name: cyclin dependent kinase inhibitor 2A; targeted mutation 3, Charles J Sherr
• MGI ID: MGI:3842628
• Synonyms: Arf-Cre
• Gene: Cdkn2a Location: Chr4:89192710-89212856 bp, - strand Genetic Position: Chr4, 42.15 cM, cytoband C3-C6
• Alliance: Cdkn2a^tm3(cre)Cjs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147752
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Cdkn2a^tm3(cre)Cjs expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cdkn2a (MGI:104738)

• Mutation details: The targeting vector contains the sequence for cre recombinase and its 3' polyadenylation signal ligated in the opposite orientation to a dual selection cassette (Neo-Tk) containing a neomycin resistance gene and a thymidine kinase gene under control of their respective 5' promoters and polyadenylation. The selection cassette was flanked by Frt sites for deletion in ES cells. Homologous recombination left the Cdkn2a promoter and 5' UTR intact, but replaced most of exon 1Beta with cre cDNA. The linearized vector with a DTA selectable marker at the 5' end was electroporated into ES cells. It was determined that removal of the Neo-TK cassette by transfection of ES cells with FLP recombinase did not result in transmission of the cre knock-in allele, so the Neo-Tk was left intact in the positive chimeras. (J:147752)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cdkn2a (mouse) Summary of all recombinase alleles driven by Cdkn2a. Comparative matrix view of recombinase activities Alliance mouse Cdkn2a gene page

Expression

In Mice Carrying this Mutation:	3 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdkn2a Mutation: 67 strains or lines available

References

• Original: J:147752 Gromley A, et al., Transient expression of the Arf tumor suppressor during male germ cell and eye development in Arf-Cre reporter mice. Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6285-90
• All: 3 reference(s)