Bdnf^tm1Bail
Targeted Allele Detail

Summary

• Symbol: Bdnf^tm1Bail
• Name: brain derived neurotrophic factor; targeted mutation 1, Bai Lu
• MGI ID: MGI:3842639
• Synonyms: BDNF-KIV, KIV^-
• Gene: Bdnf Location: Chr2:109505045-109557352 bp, + strand Genetic Position: Chr2, 56.63 cM
• Alliance: Bdnf^tm1Bail page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147571
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutation: Insertion Mutation details: An enhanced green fluorescent protein (EGFP)-STOP cassette, followed by a loxP-flanked neomycin selection cassette (neo) in reverse orientation was inserted 3' of the promoter IV exon. Promoter IV drives expression of a fusion transcript composed of GFP and the endogenous coding sequence. However, only a GFP protein is translated from this transcript because of the GFP stop codon. Kainic acid fails to induce the normal promoter IV transcript and fails to induce an increased production of the normal protein product. (J:147571, J:269842)

Generation of the Bdnf^tm1Bail allele

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bdnf Mutation: 42 strains or lines available

References

• Original: J:147571 Sakata K, et al., Critical role of promoter IV-driven BDNF transcription in GABAergic transmission and synaptic plasticity in the prefrontal cortex. Proc Natl Acad Sci U S A. 2009 Apr 7;106(14):5942-7
• All: 9 reference(s)