Tnfrsf4^tm2(cre)Nik
Targeted Allele Detail

Summary

• Symbol: Tnfrsf4^tm2(cre)Nik
• Name: tumor necrosis factor receptor superfamily, member 4; targeted mutation 2, Nigel Killeen
• MGI ID: MGI:3843034
• Synonyms: Ox40-Cre
• Gene: Tnfrsf4 Location: Chr4:156098300-156101069 bp, + strand Genetic Position: Chr4, 87.68 cM
• Alliance: Tnfrsf4^tm2(cre)Nik page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147746
• Parent Cell Line: JM-1 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Tnfrsf4^tm2(cre)Nik expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tnfrsf4 (MGI:104512)

• Mutation details: 1.7 kb of the locus containing exons 1, 2 and part of 3 was fused to an IRES-cre construct followed by a FRT-flanked neomycin selection element. After targeted recombination, the neomycin element was removed by crossing founder mice with FLPe transgenic mice. Insertion of the cre expression construct disrupted the endogenous locus, creating a null allele. Cre expression was detected in male but not female germline cells, in memory and regulatory CD4⁺ T cells, and in some CD8⁺ and naive CD4⁺ T cells. (J:147746)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tnfrsf4 (mouse) Summary of all recombinase alleles driven by Tnfrsf4. Alliance mouse Tnfrsf4 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tnfrsf4 Mutation: 36 strains or lines available

References

• Original: J:147746 Klinger M, et al., Thymic OX40 expression discriminates cells undergoing strong responses to selection ligands. J Immunol. 2009 Apr 15;182(8):4581-9
• All: 31 reference(s)