Tcrb^tm1(Tcrb)Chb
Targeted Allele Detail

Summary

• Symbol: Tcrb^tm1(Tcrb)Chb
• Name: T cell receptor beta chain; targeted mutation 1, Craig H Bassing
• MGI ID: MGI:3843491
• Synonyms: Jbeta1^DJbeta
• Gene: Tcrb Location: Chr6:40868230-41535305 bp, + strand Genetic Position: Chr6, cytoband A-C

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147947
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intergenic deletion
• Mutation details: ES cells homozygous for the Tcrb^tm1Slek allele that lacks the Dbeta2-Jbeta2 was targeted a second time. A preassembled Dbeta1Jbeta1.1 complex, which did not contain N or P nucleotides and was missing just one C nucleotide compared with the genomic sequence, replaced the germline Dbeta1 and Jbeta1.1 segments. A floxed neomycin resistance gene and a HindIII restriction site were inserted between the Jbeta1.2 and Jbeta1.3 segments. Transient expression of cre recombinase removed the neomycin cassette while leaving behind the HindIII site and a loxP site. The novel HindIII site helped distinguish between Tcrb rearrangements on this allele versus the Tcrb^tm1Slek allele. (J:147947)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcrb Mutation: 97 strains or lines available

References

• Original: J:147947 Carpenter AC, et al., Assembled DJbeta complexes influence TCRbeta chain selection and peripheral Vbeta repertoire. J Immunol. 2009 May 1;182(9):5586-95
• All: 4 reference(s)