Hvcn1^{Gt(RRN293)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Hvcn1^{Gt(RRN293)Byg}
• Name: hydrogen voltage-gated channel 1; gene trap RRN293, BayGenomics
• MGI ID: MGI:3843777
• Synonyms: Hv1, RPN293
• Gene: Hvcn1 Location: Chr5:122344872-122380360 bp, + strand Genetic Position: Chr5, 62.23 cM
• Alliance: Hvcn1^{Gt(RRN293)Byg} page

Mutation origin

• Mutant Cell Line: RRN293
• Germline Transmission: Earliest citation of germline transmission: J:148032
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The gene trap vector, which contains a beta-geo cassette, inserted into intron 2 (J:148273 and J:158627) or between exons 3 and 4 (J:148032) depending on the gene model used. The absence of protein expression was confirmed by western blot analysis on macrophage and neutrophil extracts. (J:148032, J:148273, J:158627)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRN293	CG671904 ; (MGI Seq Detail)	5' RACE	Chr5:122344900-122354423 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  22 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hvcn1 Mutation: 30 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:148032 Okochi Y, et al., Voltage-gated proton channel is expressed on phagosomes. Biochem Biophys Res Commun. 2009 May 1;382(2):274-9
• All: 30 reference(s)