Prss16^tm1.1Mal
Targeted Allele Detail

Summary

• Symbol: Prss16^tm1.1Mal
• Name: serine protease 16 (thymus); targeted mutation 1.1, Bernard Malissen
• MGI ID: MGI:3843929
• Synonyms: Prss16^tm1Mal, TSSP^-, Tssp^o
• Gene: Prss16 Location: Chr13:22186346-22193911 bp, - strand Genetic Position: Chr13, 8.12 cM, cytoband A3-A5
• Alliance: Prss16^tm1.1Mal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:148034
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exons 8-12 of the locus were replaced by a self-excising floxed neomycin and cre recombinase expression cassette. Genomic PCR on homozygotes confirmed deletion of both the exons and the selection cassette. Northern and Western blots on homozygote thymi extracts confirmed gene inactivation. (J:148034)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Prss16 Mutation: 41 strains or lines available

References

• Original: J:148034 Gommeaux J, et al., Thymus-specific serine protease regulates positive selection of a subset of CD4+ thymocytes. Eur J Immunol. 2009 Apr;39(4):956-64
• All: 8 reference(s)