Itgb3^tm2Aja
Targeted Allele Detail

Summary

• Symbol: Itgb3^tm2Aja
• Name: integrin beta 3; targeted mutation 2, Ararat J Ablooglu
• MGI ID: MGI:3844537
• Synonyms: Beta3/Beta1
• Gene: Itgb3 Location: Chr11:104498826-104561302 bp, + strand Genetic Position: Chr11, 67.84 cM
• Alliance: Itgb3^tm2Aja page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:148306
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: The sequence encoding the final three C-terminal residues (arginine-glycine-threonine) that are necessary for interaction with Src kinase were replaced with sequence encoding cysteine-glycine-lysine (EGK) isolated from the Itgb1 locus. This mutation was predicted to be interact with other Src family protein tyrosine kinases. Targeting constructs also contained several silent mutations to introduce new HpaI and NdeI restriction sites and a floxed neomycin-resistance cassette between exons 14 and 15. Mice carrying the correctly targeted allele were crossed with cre transgenic mice to remove the neomycin selection cassette. Extracellular protein expression levels on homozygous platelets was 125% of wild-type levels. (J:148306)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Itgb3 Mutation: 45 strains or lines available

References

• Original: J:148306 Ablooglu AJ, et al., Antithrombotic effects of targeting alphaIIbbeta3 signaling in platelets. Blood. 2009 Apr 9;113(15):3585-92
• All: 2 reference(s)