Agr2^tm1.2Erle
Targeted Allele Detail

Summary

• Symbol: Agr2^tm1.2Erle
• Name: anterior gradient 2; targeted mutation 1.2, David J Erle
• MGI ID: MGI:3844570
• Gene: Agr2 Location: Chr12:36042924-36054080 bp, + strand Genetic Position: Chr12, 16.18 cM
• Alliance: Agr2^tm1.2Erle page

Agr2^tm1.2Erle/Agr2^tm1.2Erle mice are devoid of intestinal mucus

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:148339
• Parent Cell Line: RF8 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: Exons 2 and 3 were floxed by insertion of a loxP site upstream of exon 2 and the addition of a FRT-neo-loxP-FRT-loxP cassette downstream of exon 3. The neo cassette was subsequently removed from correctly targeted ES cells by transient expression of FLPe recombinase. Mice carrying the floxed allele were crossed with transgenic mice that express cre recombinase in the germline to create a null allele. Gene inactivation was confirmed by RT-PCR analysis on intestinal extracts. (J:148339)

Generation of the Agr2^tm1.2Erle allele

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Agr2^tm1.2Erle

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Agr2 Mutation: 34 strains or lines available

References

• Original: J:148339 Park SW, et al., The protein disulfide isomerase AGR2 is essential for production of intestinal mucus. Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):6950-5
• All: 9 reference(s)