Cpn1^tm1Raw
Targeted Allele Detail

Summary

• Symbol: Cpn1^tm1Raw
• Name: carboxypeptidase N, polypeptide 1; targeted mutation 1, Rick A Wetsel
• MGI ID: MGI:3844631
• Gene: Cpn1 Location: Chr19:43944746-43974990 bp, - strand Genetic Position: Chr19, 36.68 cM, cytoband D2
• Alliance: Cpn1^tm1Raw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:148315
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S1/SvImJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 3 of the locus was replaced by a neomycin resistance cassette. RT-PCR analysis on homozygous liver extracts detected the presence of a truncated mRNA message corresponding to a splice variant that fused exon 2 to exon 4. Because exon 3 encoded a substrate binding site, it was hypothesized that any mutant protein transcribed from the truncated mRNA would lack carboxypeptidase activity. This was confirmed by a lack of terminal arginine cleavage on C3a and C5a complement proteins. (J:148315)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cpn1 Mutation: 18 strains or lines available

Notes

ES cell line = EZ-1

References

• Original: J:148315 Mueller-Ortiz SL, et al., Targeted disruption of the gene encoding the murine small subunit of carboxypeptidase N (CPN1) causes susceptibility to C5a anaphylatoxin-mediated shock. J Immunol. 2009 May 15;182(10):6533-9
• All: 1 reference(s)