Gt(ROSA)26Sor^{tm2(Nfatc2*)Rao}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(Nfatc2*)Rao}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Anjana Rao
• MGI ID: MGI:3844635
• Synonyms: R26^{STOPflox-AV-NFAT1}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(Nfatc2*)Rao} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:148341
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm2(Nfatc2*)Rao} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Nfatc2 (MGI:102463)

• Mutation details: A construct consisting of a splice acceptor site, floxed neo-stop cassette, hyperactivatable Nfatc2 gene, and an frt flanked IRES-GFP cassette was inserted into intron 1 via homologous recombination. The hyperactivatable Nfatc2 gene contains mutations in the calcineurin docking site resulting in higher affinity for ligand and mutations in the CK1 docking site resulting in lower affinity for the ligand. The mutant protein sequences for the calcineurin site and the CK1 site are HPVIVITGP and ASILA, respectively. (J:148341)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:148341 Muller MR, et al., Requirement for balanced Ca/NFAT signaling in hematopoietic and embryonic development. Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):7034-9
• All: 2 reference(s)