Dclk2^tm1.2Jgg
Targeted Allele Detail

Summary

• Symbol: Dclk2^tm1.2Jgg
• Name: doublecortin-like kinase 2; targeted mutation 1.2, Joseph G Gleeson
• MGI ID: MGI:3844726
• Gene: Dclk2 Location: Chr3:86693458-86828159 bp, - strand Genetic Position: Chr3, 37.88 cM
• Alliance: Dclk2^tm1.2Jgg page

Hippocampal disorganization, increased c-fos, and loss of SOM+ interneurons in Dcx^tm1Caw/Y Dclk2^tm1.2Jgg/Dclk2^tm1.2Jgg mice

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:148345
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted (Null/knockout) Mutation: Intragenic deletion Mutation details: A loxP site was inserted upstream of exon 2 and a floxed neomycin selection cassette was inserted downstream of the exon. Exon 2 was removed by crossing founder mice with transgenic mice that express cre recombinase in the germline removed exon 2, and led to an out-of-frame mutation and the appearance of a stop codon within exon 3. Immunoblot analysis indicated a reduction in protein expression in heterozygote brain, and absence of protein in homozygote brain. (J:148345)

Generation of the Dclk2^tm1Jgg and Dclk2^tm1.2Jgg alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dclk2 Mutation: 135 strains or lines available

References

• Original: J:148345 Kerjan G, et al., Mice lacking doublecortin and doublecortin-like kinase 2 display altered hippocampal neuronal maturation and spontaneous seizures. Proc Natl Acad Sci U S A. 2009 Apr 21;106(16):6766-71
• All: 1 reference(s)