Hras^tm1Jaf
Targeted Allele Detail

Summary

• Symbol: Hras^tm1Jaf
• Name: Harvey rat sarcoma virus oncogene; targeted mutation 1, James A Fagin
• MGI ID: MGI:3845022
• Synonyms: FR-Hras^G12V, Hras1^G12V, Hras^G12V
• Gene: Hras Location: Chr7:140770839-140773938 bp, - strand Genetic Position: Chr7, 86.48 cM
• Alliance: Hras^tm1Jaf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:148393
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The targeted locus consisted of 2 tandemly-arrayed murine Hras1 genes with the upstream wild-type copy flanked by loxP sites and the downstream copy containing a glycine-to-valine mutation at amino acid 12 (G12V). Between the two genes was placed a SV40 poly A sequence and a FRT-flanked neomycin selection cassette that was subsequently removed by crossing with transgenic mice expressing the FLPe recombinase. In the absence of cre expression, only the wild-type gene is expressed. In the presence of cre recombinase, the wild-type gene is excised and the mutant gene is instead expressed. (J:148393)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	7 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Hras^tm1Jaf

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hras Mutation: 30 strains or lines available

Notes

Phenotypic Similarity to Human Syndrome: Thyroid Cancer J:231492 in conditional mice homozygous for Hras^tm1Jaf and homozygous for either Nf2^tm2Gth, Trp53^tm1Brn or Pten^tm2.1Ppp.

References

• Original: J:148393 Chen X, et al., Endogenous expression of Hras(G12V) induces developmental defects and neoplasms with copy number imbalances of the oncogene. Proc Natl Acad Sci U S A. 2009 May 12;106(19):7979-84
• All: 17 reference(s)