Nihls1^CAST/EiJ
QTL Variant Detail

Summary

• QTL variant: Nihls1^CAST/EiJ
• Name: noise-induced hearing loss susceptibility 1; CAST/EiJ
• MGI ID: MGI:3845108
• QTL: Nihls1 Location: unknown Genetic Position: Chr2, Syntenic

Variant origin

• Strain of Specimen: CAST/EiJ

Variant description

• Allele Type: QTL
• Mutation: Undefined
• Mutation details: This allele confers decreased recovery from inital noice-induced hearing damage compared to C57BL/6J. (J:147452)

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:147452

A series of genome-tagged congenic lines derived from CAST/EiJ donor DNA segments on a C57BL/6J genetic background were used to map genetic loci involved in noise-induced hearing loss. The genome-tagged mice consist of 39 chromosome-specific lines covering approximately 80%of the genome. Animals were exposed to 108 dB sound pressure level (SPL) intensity noise at 6-8 weeks of age and assessed for hearing 23 days later via auditory brainstem response (ABR). Parental strain C57BL/6J is susceptible to noise-induced hearing loss whereas parental strain CAST/EiJ is resistant. Pre-exposure thresholds were similar between parental strains.

Genome-tagged congenic line B6.CAST.4PM displayed permanent threshold shift at one-third the level of parental strain C57BL/6J. B6.CAST.4PM carries CAST/EiJ-derived donor DNA from 0 cM to 67 cM (between rs13477534 and rs6355453) which localizes a resistance QTL for noise-induced hearing loss to this interval. CAST/EiJ alleles at this locus confer enhanced recovery from initial hearing damage and the QTL is named Nihlr1 (noise-induced hearing loss resistance 1). Animals from congenic line B6.CAST.4PM display almost complete recovery 23 days after noise-induced damage. Fbxo2 at 147.5 Mb (71 cM) is located near the Nihlr1 interval and is potentially a candidate gene. More analysis is required to identify candidate genes as the QTL interval is large.

Genome-tagged congenic line B6.CAST.2P displayed decreased recovery from noise-induced hearing loss compared to C57BL/6J. Thiscongenic line carries CAST/EiJ-derived alleles at the proximal portion of chromosome 2 on a C57BL/6J genetic background indicating this region confers susceptibility to initial noise-induced hearing damage. This QTL is designated Nihls1 (noise-induced hearing loss susceptibility 1). Animals from B6.CAST.2P congenic line displays minimal recovery 23 days after noise-induced damage. The precise map location for the CAST/EiJ-derived congenic interval in B6.CAST.2P is not specified.

References

• Original: J:147452 White CH, et al., Genome-wide screening for genetic loci associated with noise-induced hearing loss. Mamm Genome. 2009 Apr;20(4):207-13
• All: 1 reference(s)