Pitgp^C57BL/6
QTL Variant Detail

Summary

• QTL variant: Pitgp^C57BL/6
• Name: Pecam1-independent thioglycollate peritonitis; C57BL/6
• MGI ID: MGI:3845114
• QTL: Pitgp Location: unknown Genetic Position: Chr2, Syntenic

Variant origin

• Strain of Specimen: C57BL/6

Variant description

• Allele Type: QTL
• Mutation: Undefined
• Mutation details: This allele confers Pecam1-independent thioglycollate-induced inflammatory response compared to FVB/N. (J:144817)
• Inheritance: Dominant

Notes

Pecam1 knockout animals usually display impaired inflammatory response; however, genetic factors from a C57BL/6 genetic background attenuate this defect.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:144817

The Pecam1^tm1Mak knockout allele on an FVB/N genetic background results in impaired inflammatory response due to a blockade in leukocyte transendothelial migration. However, when Pecam1^tm1Mak is on a C57BL/6 genetic background inflammatory response and diapedesis are not impaired. Genetic modifiers responsible for this phenomenon were mapped by screening 150 polymorphic marker in a population of (B6.129P2-Pecam1^tm1Mak x FVB/N-Pecam1^tm1Mak)F2 intercross animals. Inflammatory response was measured after thioglycollate administration.

Significant linkage to inflammatory response mapped to proximal mouse Chromosome 2 at 28 cM (38.2 Mb) near D2Mit7 (LOD=7.5). This locus is designated Pitgp (Pecam1-independent thioglycollate peritonitis). Additional analysis of Pitgp using medium-density mapping refined the QTL location to 35.8 Mb and increased the LOD score to 9.0. Four potential candidate genes are located near Pitgp: Ptgs1 (29 cM), Ptges (24 cM), Ptges2 and Hc (23.5 cM). C57BL/6-derived alleles atPitgp confer Pecam1-independent inflammatory response in a thioglycollate model of peritonitis, and appears to exhibit dominant inheritance. Authors speculate the gene responsible for Pitgp is a gain-of-function variant.

References

• Original: J:144817 Seidman MA, et al., PECAM-independent thioglycollate peritonitis is associated with a locus on murine chromosome 2. PLoS One. 2009;4(1):e4316
• All: 1 reference(s)