Tg(Six2-EGFP/cre)1Amc
Transgene Detail

Summary

• Symbol: Tg(Six2-EGFP/cre)1Amc
• Name: transgene insertion 1, Andrew P McMahon
• MGI ID: MGI:3845228
• Synonyms: Six2Cre, Six2-TGC(tg), Six2-TGC^tg, Tet-off-eGFPCre BAC transgenic, TGC BAC transgenic allele, Tg(Six2-tTA,tetO-EGFP/cre)1Amc
• Transgene: Tg(Six2-EGFP/cre)1Amc Location: unknown
• Alliance: Tg(Six2-EGFP/cre)1Amc page

Transgene origin

• Strain of Origin: CD-1

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Six2-EGFP/cre)1Amc expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Six2 (MGI:102778)

• Mutation details: The 181 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RPCI23-311C1, containing the entire Six2 locus (and other genes), was modified by targeting a Tet-off-eGFPCre into the ATG start site of the Six2 locus. This inserted a Tet-off cassette (tetracycline-regulated transactivator (tTA), 2xpolyA signal, tetracycline-responsive element (TRE or tetO with CMV min promoter) followed by an Enhanced Greed Fluorescent Protein/Cre Recombinase (EGFP/Cre) fusion protein coding sequence, (SV40 polyA signal), and frt-flanked kanamycin cassette. The targeted BAC sequences were further modified using FLP recombination to remove the selection cassette and linearized to remove its original vector backbone. The resulting modified BAC was microinjected into CD-1 zygotes. Sequence updates published after this transgene was created revealed an upstream Six2 ATG start site that is in-frame with the tTA; thus an apparent and unintended fusion was created with the tTA (80 amino acids potentially added) that renders transgene expression unaffected by tetracycline/doxycycline administration. (J:148455)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Six2 (mouse) Summary of all recombinase alleles driven by Six2. Alliance mouse Six2 gene page

Disease models

Expression

In Mice Carrying this Mutation:

398 assay results 17 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:148455 Kobayashi A, et al., Six2 defines and regulates a multipotent self-renewing nephron progenitor population throughout mammalian kidney development. Cell Stem Cell. 2008 Aug 7;3(2):169-81
• All: 84 reference(s)