Tg(Slc6a3-PARK2*Q311X)DXwy
Transgene Detail

Summary

• Symbol: Tg(Slc6a3-PARK2*Q311X)DXwy
• Name: transgene insertion D, X William Yang
• MGI ID: MGI:3845881
• Synonyms: Parkin-Q311X(A), Parkin-Q311X(D), Parkin-Q311X line A, Parkin-Q311X line D, tgA, TgD
• Transgene: Tg(Slc6a3-PARK2*Q311X)DXwy Location: unknown
• Alliance: Tg(Slc6a3-PARK2*Q311X)DXwy page

Transgene origin

• Strain of Origin: FVB/NJ

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Slc6a3-PARK2*Q311X)DXwy expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PRKN (5071)

  Prkn	(MGI:1355296)

• Mutation details: A 200 kb mouse bacterial artificial chromosome (BAC) RP23-408F13 containing the entire 50 kb Slc6a3 gene (solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; also called dopamine transporter (DAT)) and other genes in the approximately 100 kb of 5' and 40 kbp of 3' flanking sequences, was modified by inserting a parkin-Q311X cDNA sequence (FLAG-tagged mutant human parkin gene (PARK2; Parkinson disease (autosomal recessive, juvenile) 2, parkin) carrying the Q311X truncation associated with Turkish early-onset Parkinson's disease and followed by a polyA signal) into Slc6a3 exon 2 upstream of the translation initiation codon. This modified Slc6a3-parkin(Q311X) BAC was microinjected into fertilized FVB/NJ zygotes. Founder line D (found to harbor a single copy of the transgene) was established. Median expression of the human PARK2 is substantially lower than in line A. (J:146833)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:146833 Lu XH, et al., Bacterial artificial chromosome transgenic mice expressing a truncated mutant parkin exhibit age-dependent hypokinetic motor deficits, dopaminergic neuron degeneration, and accumulation of proteinase K-resistant alpha-synuclein. J Neurosci. 2009 Feb 18;29(7):1962-76
• All: 1 reference(s)