Rela^tm2.1Mpa
Targeted Allele Detail

Summary

• Symbol: Rela^tm2.1Mpa
• Name: v-rel reticuloendotheliosis viral oncogene homolog A (avian); targeted mutation 2.1, Manolis Pasparakis
• MGI ID: MGI:3846176
• Synonyms: GFP-p65, p65^GFP-FL
• Gene: Rela Location: Chr19:5687511-5698158 bp, + strand Genetic Position: Chr19, 4.34 cM, cytoband B1-3
• Alliance: Rela^tm2.1Mpa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:148519
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

Allele Type: Targeted (Conditional ready, No functional change) Mutation: Insertion Mutation details: An enhanced GFP coding cassette was knocked into exon 1 of the locus immediately downstream of the first ATG of the open reading frame. A FRT-flanked neomycin cassette and a loxP site were inserted upstream of exon 2, and an additional loxP site was inserted downstream of exon 4. Mice were crossed with cre-deleter mice to remove the neomycin cassette. Homozygous mice expressed a fluorescent eGFP-Rela fusion protein as determined by immunoblot and flow cytometry analyses on spleen, thymus and lymph node cells. Endogenous function of the locus was unaffected as determined by several biochemical and phenotypical analyses. (J:148519)

Generation of the Rela^tm2.1Mpa allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rela Mutation: 28 strains or lines available

References

• Original: J:148519 De Lorenzi R, et al., GFP-p65 knock-in mice as a tool to study NF-kappaB dynamics in vivo. Genesis. 2009 Mar 4;47(5):323-329
• All: 7 reference(s)