Tg(Scgb1a1-rtTA,-tTS,tetO-CHI3L1)1Eli
Transgene Detail

Summary

• Symbol: Tg(Scgb1a1-rtTA,-tTS,tetO-CHI3L1)1Eli
• Name: transgene insertion 1, Jack A Elias
• MGI ID: MGI:3846185
• Synonyms: CC10-rtTA-tTS-YKL-40
• Transgene: Tg(Scgb1a1-rtTA,-tTS,tetO-CHI3L1)1Eli Location: unknown
• Alliance: Tg(Scgb1a1-rtTA,-tTS,tetO-CHI3L1)1Eli page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(Scgb1a1-rtTA,-tTS,tetO-CHI3L1)1Eli expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CHI3L1 (1116)

  Chi3l1

  (MGI:1340899)

• Mutation details: Three constructs were co-injected to create this transgene. Two constructs used the rat Scgb1a1 (CC10) promoter to drive either reverse tetracycline transactivator (rtTA) or a tetracycline-controlled transcriptional silencer (tTS). In this system, the CC10 promoter constitutively drives the expression of rtTA and tTS in a lung-specific fashion. In the absence of doxycycline, tTS binds to and actively suppresses the expression of the tet-O-regulated human CHI3L1 transgene. In the presence of dox, tTS is released allowing the activating, dox binding rtTA to bind to the tet-O and activate transgene expression. Expression of human CHI3L1 was detected in bronchio-alveolar fluid 24 hours after doxycycline administration. (J:148490)

Generation of the Tg(Scgb1a1-rtTA,-tTS,tetO-CHI3L1)1Eli transgene

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:148490 Lee CG, et al., Role of breast regression protein 39 (BRP-39)/chitinase 3-like-1 in Th2 and IL-13-induced tissue responses and apoptosis. J Exp Med. 2009 May 11;206(5):1149-66
• All: 8 reference(s)