Col14a1^tm1Debi
Targeted Allele Detail

Summary

• Symbol: Col14a1^tm1Debi
• Name: collagen, type XIV, alpha 1; targeted mutation 1, David E Birk
• MGI ID: MGI:3846440
• Gene: Col14a1 Location: Chr15:55171146-55384199 bp, + strand Genetic Position: Chr15, 21.96 cM, cytoband D
• Alliance: Col14a1^tm1Debi page

Altered fibroblast and fiber organization in P4 tendons from Col14a1^tm1Debi/Col14a1^tm1Debi mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:148619
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Nucleotide substitutions Mutation details: A nonsense mutation was generated by inserting a stop codon followed by a neo-TK cassette into exon 4. Immunoblot analysis confirmed the absence of protein expression in the skin of homozygous mice at P12 and P60 (J:148619)

Creation of the Col14a1^tm1Debi allele

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col14a1 Mutation: 80 strains or lines available

References

• Original: J:148619 Ansorge HL, et al., Type XIV Collagen Regulates Fibrillogenesis: PREMATURE COLLAGEN FIBRIL GROWTH AND TISSUE DYSFUNCTION IN NULL MICE. J Biol Chem. 2009 Mar 27;284(13):8427-38
• All: 3 reference(s)