Hprt1^{tm1(CAG-Tgfbr1*)Lba}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm1(CAG-Tgfbr1*)Lba}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Laurent Bartholin
• MGI ID: MGI:3846738
• Synonyms: LSL-TbetaRI^CA, Stop^floxTgfbRI^CA, TbetaRI^CA, TGFbetaRI^CA
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm1(CAG-Tgfbr1*)Lba} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:148747
• Parent Cell Line: BPES (ES Cell)
• Strain of Origin: (129-Hprt1^b-m3 x C57BL/6)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Constitutively active, Inserted expressed sequence)
• Mutation: Insertion
• Hprt1^{tm1(CAG-Tgfbr1*)Lba} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tgfbr1 (MGI:98728)

• Mutation details: Coding sequence for a constitutively active form of murine TGFbeta receptor I was placed downstream of a CAG promoter and a loxP-STOP-loxP motif. An HA tag was included on the C-terminus end followed by the first three exons of the Hprt locus to allow for correct targeting and selection in ES cells that had a deletion of the 5' end of the Hprt locus. The knocked-in Tgfbr1 sequence encoded three missense mutations: T204D that constitutively activates the TbRI kinase and L193A/P194A , mutations that prevent binding of the TbRI inhibitor, FKBP12. Expression of the gene can only be achieved by cre-mediated removal of the floxed STOP cassette. (J:148747)

Tumor Data

List all tumor models in MMHCdb carrying Hprt1^{tm1(CAG-Tgfbr1*)Lba}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

References

• Original: J:148747 Bartholin L, et al., Generation of mice with conditionally activated transforming growth factor beta signaling through the TbetaRI/ALK5 receptor. Genesis. 2008 Dec;46(12):724-31
• All: 17 reference(s)