Pds5a^{Gt(RRM243)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Pds5a^{Gt(RRM243)Byg}
• Name: PDS5 cohesin associated factor A; gene trap RRM243, BayGenomics
• MGI ID: MGI:3847257
• Synonyms: Pds5A^-
• Gene: Pds5a Location: Chr5:65763062-65855579 bp, - strand Genetic Position: Chr5, 33.76 cM, cytoband C3.3
• Alliance: Pds5a^{Gt(RRM243)Byg} page

Pds5a^{Gt(RRM243)Byg}/Pds5a^{Gt(RRM243)Byg} mice display growth retardation, abnormal skeletal patterning and cleft pallet

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: RRM243
• Germline Transmission: Earliest citation of germline transmission: J:148890
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The pGT0lxf vector, containing a beta-geo cassette, inserted into intron 2 and disrupted normal gene splicing. The absence of wild-type protein expression was confirmed by western blot analysis on E18.5 kidney extracts. (J:148890)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRM243	CG672109 ; (MGI Seq Detail)	5' RACE	Chr5:65854107-65855184 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  54 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Generation of the Pds5a^{Gt(RRM243)Byg} allele

Disease models

Expression

In Structures Affected by this Mutation:

10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pds5a Mutation: 81 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:148890 Zhang B, et al., Dosage effects of cohesin regulatory factor PDS5 on mammalian development: implications for cohesinopathies. PLoS One. 2009;4(5):e5232
• All: 2 reference(s)