Nags^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Nags^{tm1(KOMP)Vlcg}
• Name: N-acetylglutamate synthase; targeted mutation 1, Velocigene
• MGI ID: MGI:3847452
• Gene: Nags Location: Chr11:102036339-102040303 bp, + strand Genetic Position: Chr11, 65.48 cM
• Alliance: Nags^{tm1(KOMP)Vlcg} page
• IMPC: Nags gene page

Mutation origin

• Mutant Cell Lines: 10229B-H2, 10229E-B5
• Germline Transmission: Earliest citation of germline transmission: J:184448
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 3502bp between positions 102036396-102039897 of Chromosome 11 (Build GRCm39). Western blot analysis confirmed the absence of protein expression in the liver and intestines of homozygous mice. (J:136110, J:184448)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Nags Mutation: 27 strains or lines available

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 4 reference(s)