Eif3f^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Eif3f^{tm1(KOMP)Vlcg}
• Name: eukaryotic translation initiation factor 3, subunit F; targeted mutation 1, Velocigene
• MGI ID: MGI:3847510
• Synonyms: eIF3f^-
• Gene: Eif3f Location: Chr7:108533622-108541149 bp, + strand Genetic Position: Chr7, 57.21 cM
• Alliance: Eif3f^{tm1(KOMP)Vlcg} page
• IMPC: Eif3f gene page

Mutation origin

• Mutant Cell Lines: 11510A-F5, 11510B-A4, 11510B-B5, 11510B-B7, 11510B-C10, 11510B-E5, 11510B-H5
• Germline Transmission: Earliest citation of germline transmission: J:294674
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 7130bp between positions 108533623-108540752 of Chromosome 7 (Build GRCm39). Real-time PCR and Western blot analyses revealed a ~50% reduction of mRNA and protein levels in all adult heterozygous tissues/organs examined. Quantification of immunoblots indicated that protein expression levels are decreased by 30-46% in adult heterozygous skeletal muscles. (J:136110)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Eif3f Mutation: 22 strains or lines available

Notes

Cell lines 11510A-F5 (found to be 61% euploid), 11510B-A4 (>75% euploid) and 11510B-C10 (>75% euploid) were successfully used to make chimeric mice. Germline transmission was accomplished for all three lines. J:294674

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 2 reference(s)