Elovl5^{Gt(DTM098)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Elovl5^{Gt(DTM098)Byg}
• Name: ELOVL fatty acid elongase 5; gene trap DTM098, BayGenomics
• MGI ID: MGI:3847980
• Synonyms: Elovl5^-
• Gene: Elovl5 Location: Chr9:77824647-77891801 bp, + strand Genetic Position: Chr9, 43.36 cM
• Alliance: Elovl5^{Gt(DTM098)Byg} page

Mutation origin

• Mutant Cell Line: DTM098
• Germline Transmission: Earliest citation of germline transmission: J:149093
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector Vector: pGT1dTMpfs Vector Type: secretory trap
• Mutation details: A genetrap vector inserted 7.9 kb downstream of exon 1, which was upstream of the ATG translational start site located in exon 2. Transcript levels were reduced by more than 99% as determined by Northern blot analysis on homozygote liver extracts. (J:149093)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  DTM098	CC156103 ; (MGI Seq Detail)	5' RACE	unknown
  * GRCm39; (strand of gene trap mutagenesis)

Generation of the Elovl5^{Gt(DTM098)Byg} gene trap allele

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Elovl5 Mutation: 94 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:149093 Moon YA, et al., Deletion of ELOVL5 leads to fatty liver through activation of SREBP-1c in mice. J Lipid Res. 2009 Mar;50(3):412-23
• All: 6 reference(s)